Identificação e caracterização de genes codificantes de quitinase no genoma de Handroanthus impetiginosus

Abstract

Chitinases are enzymes capable of hydrolyzing chitins, which are the second most abundant polymer on the planet, present in insect exoskeleton and fungal cell wall. Chitinases in plants are divided into two families of glycosyl hydrolases, families 18 and 19, and subdivided into classes I, II, III, IV and V. These enzymes have their main function related to protection against chitin-containing insects and pathogens. composition. Therefore, this trait can be used, with the help of genetic engineering, to generate pest resistant plants. Currently, genomics and bioinformatics make it possible to identify these resistance genes from the plant genome. The pink ipe (Handroanthus impetiginosus) is known for its great insect resistance. Therefore, the objective of this work was the identification and characterization of chitinases genes in the Handroanthus impetiginosus genome. Using the BLASTp, Blast2GO and MEGAX programs, a search for chitinase genes from families 18 and 19 in the H. impetiginosus genome was performed. These sequences were then confirmed to have family 18 or 19 glycosyl hydrolase catalytic domains. These sequences were aligned and analyzed by phylogenetic trees to classify these chitinases into the five known classes. The WoLF PSORT site was used to indicate the possible cellular location of chitinases and SWISS-MODEL was used to predict 3D structures. The searches resulted in 12 family 18 genes and 15 family 19 genes, all featuring the 3D domains and structure characteristic of chitinases.