Análise histopatológica renal de neonatos de Podocnemis unifilis (TESTUDINES, Podocnemididae) expostos ao Fipronil e ao Glifosato durante a incubação dos ovos

Abstract

The progress in agricultural activity is associated with the increase agrochemicals utilization, which insures the quality products once there is an augment on demand for agriculture products. The pesticides have been used indiscriminately resulting in water resources contamination, and endangering of several aquatic species. Studies conducted with amphibians and reptiles have been evaluating the ecotoxicity of pesticides, reinforcing the importance of understanding how commercial formulations may be affecting the natural habitat and even the development of these animals. Therefore, the present work aims to evaluate the renal histopathology in newborns of Tracajá (Podocnemis unifilis) exposed to pesticides (Glyfosate and Fipronil) during incubation period. The animals were subjected to physical restraint and subsequent euthanasia, followed by the obtainment of the kidneys, with 10 % formalin fixation and processed according to the histological routine. Through the histological analysis, we observed and inferred distortions in the glomerular area and the urinary space of animals exposed to pesticides. All groups exposed to pesticides presented larger glomerular area and urinary space compared to the control group. Furthermore, fatty degeneration was observed in kidneys of the animals exposed to Fipronil.