Efeito dos meios de armazenamento na composição química da dentina humana

Abstract

The aim of this study was to evaluate the effect of different teeth storage media for in vitro studies on dentin chemical composition. Seventy healthy third molars were selected and divided into 7 groups according to the type of storage (n= 10) : CT Group , samples prepared and analyzed within 24 hours of extraction , not being stored in any medium; DI group , samples stored in distilled water; DE group , samples stored in deionized water; AU Group, samples stored in ultrapure water; SS Group, samples stored in saline; SA Group , samples stored in artificial saliva; AD Group, frozen samples at - 20 C. The teeth were cut out and after 30 days and 90 days of storage the samples were analyzed using Fourier Transform Infrared Spectroscopy (FTIR / ATR) by means of reasons: phosphate / amide I (M : M) ; carbone / phosphate (C: M); and amide I / amide III. The data were analyzed with the One-way ANOVA parametric test , then submitted to Dunnett's test (α = 0.05) and Within each experimental group, a paired T-test was performed to compare the two storage times. In the FTIR, there was a significant difference for the C: M, M: M ratios and the amide I / amide III in all samples stored in their respective media. It follows that d ifferent means and time storage of the can hange the chemical properties of the dentin, changing their portions organics and inorganics .