Identificação de polimorfirmos entre cafeeiros sensíveis e tolerantes a seca utilizando marcadores AFLP

Abstract

The coffee tree is a well distributed plant by the planet and constitutes one of the main agricultural products in Brazil making him occupy spotlight positions in production and consumption.However, the high demand of nutrients and water makes this organism presents stress conditions which contribute for quite significant falls in the production.Thereby,the vegetable developed morphological and molecular characters to deal with this scenario, which are explored for breeding. For the selection and obtainment of cultivars with agronomic traits of interest is used phenotypic and molecular markers.Thus, the present work objected the obtainment hybrids that are drought tolerant, leaf rust resistant and holders of beverage quality by crossing Siriema AS1 and Catiguá MG2 parental, as well as the obtainment of the polymorphisms between them trough the AFLP technique. The crosses were satisfactory with the germination of 78 in 92 obtained seeds and the DNA quality was determined by reading in spectrophotometer and running in 0.8% agarose gel. The gel analysis after the amplification with four primers combinations (E-AAC/M-CAA, E-ACT/M-CAA, E-ACC/M-CAG e E-AAC/M-CTG) revealed 14 polymorphic bands of a total of 82 amplified bands, which may be related with characters that differ the parental, among them the drought stress tolerance. The association of polymorphic bands with the drought tolerance demands the advancement of generations and the analysis of the plants in the segregating population as drought stress tolerance.