Avaliação da atividade de alcalóides sintéticos no ciclo replicativo do zikv e chikv in vitro

Abstract

Chikungunya (CHIKV) virus and Zika (ZIKV) are arboviruses transmitted by the bite of female mosquitoes of Aedes genus. In the last years has been an increase in the number of infected people by these viruses and the number of contries that notifed cases of Chikungunya and Zika fever. There is not yet available vaccine or antiviral to treat patients infected with these viruses, there is the need of the study of molecules with potential antiviral activity against Arboviruses. This study evaluated the antiviral activity of 9 synthetic alkaloids in replication of CHIKV in cell line expressing subgenomic replicon, wich expressed non-structural proteins of CHIKV and reporters Renilla and EGFP. In addition, also evaluated the antiviral octivity of these synthetic compounds against ZIKV infection with viral particles with repeorter Renilla. The results showed that the alkaloid Fac 2 inhibited 75.55 % of CHIKV replication, and did not showed citotoxicity to the treated cells. The synthetic alkaloids Fac 7, 16 e 20 inhibited 48, 46 e 35% of ZIKV infectivity in vitro. The results show that synthetic alkaloids are potential candidates to treat Chikungunya and Zika fever.