Avaliação da influência das espécies reativas de oxigênio durante a osteoclastogênese induzida em células da medula óssea de animais nox2-/-.

Abstract

The bone tissue is a dynamic structure that is under constant cycles of remodeling given by the activity of osteoblasts and osteoclasts. Most bone diseases are associated with excessive osteoclastic activity, which promote imbalance in bone remodeling, resulting in damage to the human skeleton such as those seen in osteoporosis, periodontal disease, and rheumatoid arthritis. Osteoclasts are multinucleated cells formed by the fusion of the precursors of the lineage of the bone marrow monocytes / macrophages that reach the site of reabsorption through the bloodstream. It is known that the reactive oxygen species (ROS) produced by the NADPH oxidase complex are involved in the differentiation of osteoclasts. To evaluate the influence of NOX2 on osteoclast formation, bone marrow cells were cultured from knockout mice to NOX2 against RANKL and M-CSF stimulation. The number of osteoclasts was higher in NOX2 animals compared to C57. The data are at odds with the literature since studies that inhibit the NOX2 enzyme have demonstrated that the formation of osteoclast is partially inhibited. However, there are no studies similar to this in the literature and therefore a careful investigation should be made to explain why osteoclast precursors differentiate more in NOX2 animals