Desenvolvimento de nanopartículas para incremento do sinal de espectroscopia ftir salivar como fluido diagnóstico

Abstract

The use of saliva as diagnostic fluid and disease monitoring in the medical and dental clinic can improve the quality of life indexes of the population with an earlier diagnosis and a monitoring of more frequent chronic diseases. The Fourier transform infrared spectroscopy (ATR/FTIR) allows the identification of chemical compounds efficiently without the use of reagents. However, the intensification of components measured in the FTIR by nanoparticles may increase the diagnostic potential of saliva. The hypothesis of the present study is that silver nanoparticles may interact with specific components of the saliva promoting the increase in absorbance. The present work evaluated the effect of the Ag2O (silver oxide) nanoparticle in three concentrations (1mg/ml, 0,1mg/ml, 0,01mg/ml) in the profile of saliva spectroscopy. The Saliva of healthy patients in pure form or associated with Ag2O (n=4) was evaluated by means of FTIR spectroscopy between 4000-400cm-1 using a Vertex 70 (Bruker) apparatus. The Ag2O nanoparticle at the concentration 0.01mg/ml increased (P <0.05) the vibrational mode at 3067cm-1 indicating the presence of primary and secondary amines frequently observed in proteins. Ag2O did not (P>0.05) increase the FTIR signal in the other salivary components. Thus, it was found that the application of Ag2O at the concentration of 0.01mg / ml associated with saliva can be used in diagnostic platforms that the vibrational mode 3067 cm-1 acts as salivary biomarker.