Análise da composição química do fluido crevicular gengival em dentes com hipersensibilidade dentinária através das espectroscopias ATR-FTIR e Raman

Abstract

Gingival crevicular fluid (GCF) has great potential to evidence chemical changes in different pathophysiological states. Dentin hypersensitivity (DH) is a structural and symptomatic alteration in the cervical dentinal tissue generated by a multifactorial etiology. However, chemical changes of this fluid in the DH remain undetermined. Therefore, the objective of this study was to evaluate chemical composition of GCF in DH teeth compared to with control teeth through Attenuated total reflectance Fourier transform infrared (ATR-FTIR) and Raman spectroscopy. 40 samples of GCF from DH (n = 20) and control teeth (n= 20), were collected using absorbent paper and submitted to the ATR-FTIR and Raman spectroscopy analysis. ATR-FTIR analysis presented ten distinct vibrational modes. The vibration modes 1745 cm-1 and 3020 cm-1 identified the pectin and lipid components, respectively. These presented lower concentrations (p <0.05) in the teeth with DH compared to the control. Through Raman spectroscopy were identified eight vibrational modes. The peaks at 597 cm-1 (amide VI) and 622 cm-1 (phenylalanine) were reduced (p <0.05) in the teeth with DH when compared to the control. This evidenced that DH presented chemical changes in the composition of FGC. Considering the potential functions of the altered components in the FGC, we can suggest a reduction of the mechanism of biological protection of the fluid during biocorrosive degradation. Thus, the concentration of the identified components may be a potential tool for the biological control of DH.