Estudos do efeito de nanocristais de Simonkolleite e Óxido de Zinco na produção de espécies reativas de oxigênio e citotoxicidade frente ao tratamento em linhagens tumorais de câncer de mama in vitro

Abstract

In this study, we investigated the cytotoxic effect of nanocrystals of Simonkolleite and Zinc Oxide (ZnO) as well as their nanocomposites (NCT) on tumor cells. This study is about biological experiments with MCF-10A, MCF-7 and MDA-MB-231 tumor cell lines. Nanocrystals of ZnO have an antitumor effect and are already well studied, since the nanocrystals Simonkolleite have been investigated in the area of devices and their physical and chemical effects, and no biological and oncological searchs have been found. Simonkolleite is a hydrochloride compound of zinc chloride monohydrate, naturally found in German mines. Aiming to investigate the cytotoxic effects of these single and mixed nanocrystals (composites), these nanocrystals were synthesized in the laboratory. These experiments resulted in the high incidence of cell death at high concentrations of Simonkolleite nanocrystals (NCs) and that at low concentrations the production of reactive oxygen species was observed to have the same expected effect of cytotoxicity and the production of reactive species of oxygen concentration was much higher at Zno and NCT than at the NCs at Simonkolleite. Simonkolleite induces the production of ROS in tumor cells and it seems to be associated with the apoptosis events observed in these experiments.