Caracterização por espectroscopia Raman do ácido desoxirribonucleico (DNA) de parasitos do gênero leishmania irradiados e não irradiados com radiação ionizante

Abstract

Raman spectroscopy is a very powerful tool for determining specific “molecular fingerprints” in medical applications. Its success has been strongly driven by the development of specific data analysis methods that allow us to obtain important information from the Raman spectra of complex samples such as human tissues, fluids, and tumors. Thus, this work will present the characterization of the genetic material of two parasites of the Leishmania genus, namely L. infantum - responsible of visceral leishmaniosis (VL) and L. major related of cutaneous leishmaniosis (CL). In addition, the main nitrogenous bases were characterized that make up the DNA: adenine (A), cytosine (C), guanine (G) and thymine (T), separately. This characterization was done in two phases, as before and after gamma irradiation of the samples. It was possible to attribute most of the bands present in the spectra to the bonds and chemical groups that make up the nucleic materials. The changes in the spectra after irradiation were associated with the applied gamma radiation dose.