Efeito local da associação de doxiciclina e alendronato no reparo ósseo

Abstract

The aim of the present study was to investigate the local effect of 10% doxycycline and 1% alendronate combined with poly(lactic-co-glycolic acid (PLGA) on bone repair. Thirty rats were divided into three groups, as follow: Control Group (CG), Drug Group (DG) and Vehicle-PLGA Group (VG). Bone defect was created in the right femur and filled with: blood clot (CG); PLGA gel, 10% doxycycline and 1% alendronate (DG); or vehicle-PLGA (VG). The animals were euthanized seven or fifteen days after surgery. Bone radiodensity, bone matrix and number of osteoclasts were quantified. At seven days, the findings showed increased density in DG (177.75 ± 76.5) compared with CG (80.37 ± 27.4), but no difference compared with VG (147.1 ± 41.5); no statistical difference in bone neoformation CG (25.6 ± 4.8), VG (27.8 ± 4) and DG (18.9 ± 7.8); and decrease osteoclasts in DG (4.6 ± 1.9) compared with CG (26.7 ± 7.4) and VG (17.3 ± 2.7). At fifteen days, DG (405.1 ± 63.1) presented higher density than CG (213.2 ± 60.9) and VG (283.4 ± 85.8); there was a significant increase in bone neoformation in DG (31.5 ± 4.2) compared with CG (23 ± 4), but no difference compared with VG (25.1 ± 2.9). There was decrease number of osteoclasts in DG (20.7 ± 4.7) and VG (29.5 ± 5.4) compared with CG (40 ± 9.4). The results suggest that the 10% doxycycline combination with 1% alendronate in PLGA accelerated bone repair.