Propagação in vitro e caracterização molecular de cultivares de Ricinus communis L.

Abstract

CHAPTER II: The oil extracted from castor bean seed has a lot of industrial aplications. With the creation of the National Biodiesel Program castor bean crop has been outstanding for presenting great potential for expansion in the country seeking the raw material production for biodiesel production. However, there are several aspects of crop which need to be researched for this expansion really happens in the country. Aiming to establish the in vitro germination of seeds of Ricinus communis L. and evaluate the in vitro behavior of apical buds was analyzed the action of concentrations of growth regulators NAA and BAP. The characteristics evaluated were: sprout contamination, calluses and oxidation. The germination rate is higher in seeds without the husk. Contamination rates were high. The rate of oxidation was minimized with the addition of ascorbic acid. The apical buds had production of callus and shoots the action of the hormone BAP. CHAPTER III: Due to the high global energy requirement, the search for renewable and less environmental aggressive sources of energy as in the case of biodiesel is desirable. One alternative for the production of oil might be the use of Castor oil plant (Ricinus communis L.), once it is a traditional cultivated plant by small and middle size growers, with an elevated economical and social appeal. With the recently increase in production of Castor oil plant, is necessary is the production of cultivars with genetic stability, high quality and production potential. In that sense, the existing genetic materials must be well characterized for its utilization in plant breading programs and for market supply. The conventional breeding programs are important for the selection of new parents, but the time spent to develop and launch a new variety is quite long. In this scenario, new approaches, linked to the use of molecular markers are being increasingly used in conservation programs and use of genetic resources as well as the identification, characterization and selection of promising cultivars. The aim of this study was to evaluate, using microsatellite markers, three varieties of castor beans, seeking genetic characterization. Molecular characterization were used five specific primers (primers), which did not allow distinction among the accessions from the analysis. Amplified products for SSCP.