Avaliação do controle glicêmico sanguíneo e do estresse oxidativo em pâncreas de animais diabéticos induzidos e não diabéticos tratados com extrato de Vochysia rufa e faseolamina

Abstract

Diabetes mellitus is a chronic disease that causes hyperglycemia due to deficiency in insulin production or ineffective use of the insulin that is produced. Phaseolamin is a glycoprotein extracted from Phaseolus vulgaris seeds accountable for inhibiting the activity of the enzyme alpha-amylase. Phaseolamin has been marketed and prescribed for reducing blood glucose and body weight in humans, although there are contradictions as to their effectiveness. On the other hand, Vochysia drums popularly known as \"sweet corner\" has been used in folk medicine to treat diabetes mellitus type 1 and type 2 in Uberlandia, Brazil. The mechanism of action and the phytochemical profile of the studied species is unknown. The aim of this study was to evaluate the anti-diabetic and antioxidant potential of V. rufa extract (V) and a sample of commercial phaseolamine (P) on the biochemical, antioxidants and histological parameters in non-diabetic rats (DN) and diabetic streptozotocin-induced (D) mice for 43 days. The primary drums V. phytochemical extract showed the presence of proteins and triterpenoids. The animals received water (ND control groups and D), V drums extract (500 mg / kg) (NDV and DV groups), phaseolamine (500 mg / kg) (NDP and PD group) and glyburide (6 mg / kg) (NDG and DG groups) and acarbose (25mg / kg) (NDA and DA groups) as a control drug, respectively, will gavage. DV groups, NDP, DV and DP showed no reduction in body weight compared to control groups (glibenclamide, water and acarbose). However, NDV, NDP, DV and DP, as well as NDG DG rats and reduced glucose levels compared before and after treatment. Group NDV increased urea levels (p <0.05) and aspartate aminotransferase (AST) (P <0.001) compared to control. DV compared to group D decreased the levels of AST (P <0.05), ALT (P <0.05) and HDL-C (P <0.05) as compared to group D, DG increased acid levels uric (P <0.01), creatinine (P <0.05) and decreased alkaline phosphatase levels (ALP) (P <0.01). SD rats significantly increased uric acid levels (P <0.01), creatinine (P <0.05) and urea (P <0.01) compared with mice the levels of D. The level of alkaline phosphatase (ALP) (P <0.05) decreased significantly in PD rats compared with rats D. NDP similar to the NDA increased levels of urea (P <0.01) and ALT (p <0.05). Therefore, the extracto of Vochysia was able to reduce blood glucose and alleviated the renal and hepatic effects observed in 5 streptozotocin-induced diabetic rats as well as the sample phaseolamine can cause damage to liver and kidney functions.