Avaliação da atividade antiofídica do composto p-cumarato de triacontila isolado de Bombacopsis glabra e da toxicidade aguda do extrato de Schizolobium parahyba

Abstract

The use of plant extracts as antidote against animals venoms is an old practice, mainly envenomations by snakes, besides also may used as supplemental of serum therapy. Snake venoms are a rich mixture of proteins involved in a complex series of events that depends on the synergic action of these molecules. The present study explores the ability of Triacontil p-Coumarate (PCT) an active compound isolated from root bark of Bombacopsis glabra vegetal extract (Bg) against harmful effects of Bothropoides pauloensis snake venom and isolated toxins. For assays Bg or PCT were previously incubated with venom or toxins at ratios 1:1 and 1:5 (w/w; venom/PCT) for 30 min at 37 C before the tests. Treatment condition was also assayed to simulate a snake bite with PCT inoculated at same site of venom or toxin. The PCT was able to neutralize brinogenolytic activity and plasmatic brinogen depletion induced by B. pauloensis venom or toxins tested. This compound was also able to inhibit the myotoxicity and hemorrhage caused by Bothropoides pauloensis venom or jararhagin toxin. These results were conrmed by histological studies of inoculated gastrocnemius muscle showing that jararhagin is strongly inhibited and is possible see the protective effect of PCT by larger number of regenerative cells after one week of inoculation. Our results suggest that PCT present a specicity to inhibit snake venom metalloproteinases. To determinate the possible inhibition mechanism from PCT a molecular modeling was made with docking simulations using Neuwiedase structure data. Thus, we suggest that Neuwiedase inhibition by PCT is due an interaction inside of the catalytic domain causing steric hindrance and/or disorganization of the catalytic net. In this way, PCT may provide new complementary alternative to treatments for ophidian envenomations.