1. Repositório Institucional - Universidade Federal de Uberlândia
2. Instituto de Biotecnologia (IBTEC)
3. TESE - Genética e Bioquímica

Tipo do documento:	Tese
Tipo de acesso:	Acesso Aberto
Título:	Effects of diabetes mellitus and antioxidant supplementation in oxidative stress and expression of myosin in rat brain
Título(s) alternativo(s):	Efeitos do diabetes mellitus e da suplementação com antioxidantes no estresse ocidativo e na expressão de miosinas no cérebro de ratos
Autor(es):	Calábria, Luciana Karen
Primeiro orientador:	Espindola, Foued Salmen
Primeiro membro da banca:	Taketomi, Ernesto Akio
Segundo membro da banca:	Jorge, Paulo Tannus
Terceiro membro da banca:	Antunes, Lusânia Maria Greggi
Quarto membro da banca:	Botelho, Françoise Vasconcelos
Quinto membro da banca:	Lamers, Marcelo Lazzaron
Resumo:	CHAPTER II: Ca+2/calmodulin complex interacts with and regulates various enzymes and target proteins, known as calmodulin-binding proteins (CaMBPs). This group of proteins includes molecular motors such as myosins. Non-muscle myosin-IIB was shown in our study to be overexpressed in diabetic rat brains. In this study, we compared CaMBPs, purified by immobilized-calmodulin, affinity chromatography, isolated from non-diabetic and streptozotocin-induced diabetic rat brains. Proteins eluted with EGTA and urea were separated on a SDS-PAGE gel, digested and submitted to peptide mass fingerprinting analysis. There were fifteen and sixteen intense bands for non-diabetic and diabetic brains, respectively. Two proteins were exclusively in non-diabetic brains, four were found exclusively in diabetic brains and thirteen were found in both. Protein sequences were then analyzed for the presence of the calmodulin-binding sites. A large fraction of the eluted proteins (83%) had putative IQ motifs or calmodulin-binding sites. The myosin-IIB affinity chromatography elution in association with western blot, immunohistochemistry and RT-PCR analyses strongly suggest that myosin-IIB protein and mRNA are highly expressed levels in diabetic rat brains. This is the first study that identified and showed calmodulin-binding proteins differential expression in non-diabetic and diabetic rat brains through a comparative proteomic analysis, and it opens up a new field of study relating myosin-IIB expression in the brain, diabetes mellitus and intracellular calcium regulation. CHAPTER III: Diabetes mellitus is a chronic disease characterized by persistent hyperglycemia, which may lead to brain tissue damage due to oxidative stress. The enhancement of free radicals during hyperglycemia contributes to increased neuronal death and changes in synaptic transmission. This study evaluated the effect of oxidative stress and the use of antioxidants (calcium, zinc and vitamin E) supplementation on myosins expression levels in brain tissues of streptozotocin-induced diabetes experimental rat model. Lipid peroxidation, antioxidant enzymes activities and myosins IIB and Va expressions at transcriptional and protein levels were examined after 5 and 90 days post streptozotocin induction. The chronic effect of the streptozotocininduced diabetes led to upregulation of glutathione peroxidase (GPx) and malondialdehyde (MDA) levels, and downregulation of superoxide dismutase (SOD) and catalase (CAT) activities. These alterations were accompanied by increased myosin-IIB and decreased myosin-Va expressions in diabetic rat brains. The antioxidant supplementation protected against hyperglycemia damage by reducing CAT activity and increasing GPx and SOD activities, although it had no influence on MDA levels. Interestingly, only diabetic rats restored myosin-IIB expression at both transcriptional and protein levels in brain tissues after supplementation, and no differences were observed in myosin-Va expression in comparison to non-diabetic rat brains. The functional alterations of antioxidant enzymes and myosins IIB and Va in rat brains of experimentallyinduced diabetes were strongly associated with the disease and aging during acute and chronic stages, but only myosin-IIB expression could be reversed by antioxidants. These data highlight the supplementation (calcium, zinc and vitamin E) antioxidants effects on the oxidative stress and the involvement of myosins IIB and Va in the diabetes stages; however, their mechanisms of action on enzymes activities, lipid peroxidation and myosins expression remain to be elucidated.
Palavras-chave:	Antioxidantes Miosina Diabetes mellitus Brain Calcium Calmodulin Myosin-IIB
Área(s) do CNPq:	CNPQ::CIENCIAS BIOLOGICAS::GENETICA
Idioma:	eng
País:	BR
Editora:	Universidade Federal de Uberlândia
Sigla da instituição:	UFU
Departamento:	Ciências Biológicas
Programa:	Programa de Pós-graduação em Genética e Bioquímica
Referência:	CALÁBRIA, Luciana Karen. Efeitos do diabetes mellitus e da suplementação com antioxidantes no estresse ocidativo e na expressão de miosinas no cérebro de ratos. 2010. 108 f. Tese (Doutorado em Ciências Biológicas) - Universidade Federal de Uberlândia, Uberlândia, 2010.
URI:	https://repositorio.ufu.br/handle/123456789/15714
Data de defesa:	6-Ago-2010
Aparece nas coleções:	TESE - Genética e Bioquímica

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