1. Repositório Institucional - Universidade Federal de Uberlândia
  2. Instituto de Biotecnologia (IBTEC)
  3. TESE - Genética e Bioquímica
Please use this identifier to cite or link to this item: https://repositorio.ufu.br/handle/123456789/15714
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dc.creatorCalábria, Luciana Karen
dc.date.accessioned2016-06-22T18:43:23Z-
dc.date.available2010-10-20
dc.date.available2016-06-22T18:43:23Z-
dc.date.issued2010-08-06
dc.identifier.citationCALÁBRIA, Luciana Karen. Efeitos do diabetes mellitus e da suplementação com antioxidantes no estresse ocidativo e na expressão de miosinas no cérebro de ratos. 2010. 108 f. Tese (Doutorado em Ciências Biológicas) - Universidade Federal de Uberlândia, Uberlândia, 2010.por
dc.identifier.urihttps://repositorio.ufu.br/handle/123456789/15714-
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.formatapplication/pdfpor
dc.languageengeng
dc.publisherUniversidade Federal de Uberlândiapor
dc.rightsAcesso Abertopor
dc.subjectAntioxidantespor
dc.subjectMiosinapor
dc.subjectDiabetes mellituseng
dc.subjectBraineng
dc.subjectCalciumeng
dc.subjectCalmodulineng
dc.subjectMyosin-IIBeng
dc.titleEffects of diabetes mellitus and antioxidant supplementation in oxidative stress and expression of myosin in rat braineng
dc.title.alternativeEfeitos do diabetes mellitus e da suplementação com antioxidantes no estresse ocidativo e na expressão de miosinas no cérebro de ratospor
dc.typeTesepor
dc.contributor.advisor1Espindola, Foued Salmen
dc.contributor.advisor1Latteshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4727087Y6por
dc.contributor.referee1Taketomi, Ernesto Akio
dc.contributor.referee1Latteshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788625D6por
dc.contributor.referee2Jorge, Paulo Tannus
dc.contributor.referee2Latteshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4737314J9por
dc.contributor.referee3Antunes, Lusânia Maria Greggi
dc.contributor.referee3Latteshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4728366P5por
dc.contributor.referee4Botelho, Françoise Vasconcelos
dc.contributor.referee4Latteshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794713P7por
dc.contributor.referee5Lamers, Marcelo Lazzaron
dc.contributor.referee5Latteshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4705907Z6por
dc.creator.Latteshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4776153E6por
dc.description.degreenameDoutor em Genética e Bioquímicapor
dc.description.resumoCHAPTER II: Ca+2/calmodulin complex interacts with and regulates various enzymes and target proteins, known as calmodulin-binding proteins (CaMBPs). This group of proteins includes molecular motors such as myosins. Non-muscle myosin-IIB was shown in our study to be overexpressed in diabetic rat brains. In this study, we compared CaMBPs, purified by immobilized-calmodulin, affinity chromatography, isolated from non-diabetic and streptozotocin-induced diabetic rat brains. Proteins eluted with EGTA and urea were separated on a SDS-PAGE gel, digested and submitted to peptide mass fingerprinting analysis. There were fifteen and sixteen intense bands for non-diabetic and diabetic brains, respectively. Two proteins were exclusively in non-diabetic brains, four were found exclusively in diabetic brains and thirteen were found in both. Protein sequences were then analyzed for the presence of the calmodulin-binding sites. A large fraction of the eluted proteins (83%) had putative IQ motifs or calmodulin-binding sites. The myosin-IIB affinity chromatography elution in association with western blot, immunohistochemistry and RT-PCR analyses strongly suggest that myosin-IIB protein and mRNA are highly expressed levels in diabetic rat brains. This is the first study that identified and showed calmodulin-binding proteins differential expression in non-diabetic and diabetic rat brains through a comparative proteomic analysis, and it opens up a new field of study relating myosin-IIB expression in the brain, diabetes mellitus and intracellular calcium regulation. CHAPTER III: Diabetes mellitus is a chronic disease characterized by persistent hyperglycemia, which may lead to brain tissue damage due to oxidative stress. The enhancement of free radicals during hyperglycemia contributes to increased neuronal death and changes in synaptic transmission. This study evaluated the effect of oxidative stress and the use of antioxidants (calcium, zinc and vitamin E) supplementation on myosins expression levels in brain tissues of streptozotocin-induced diabetes experimental rat model. Lipid peroxidation, antioxidant enzymes activities and myosins IIB and Va expressions at transcriptional and protein levels were examined after 5 and 90 days post streptozotocin induction. The chronic effect of the streptozotocininduced diabetes led to upregulation of glutathione peroxidase (GPx) and malondialdehyde (MDA) levels, and downregulation of superoxide dismutase (SOD) and catalase (CAT) activities. These alterations were accompanied by increased myosin-IIB and decreased myosin-Va expressions in diabetic rat brains. The antioxidant supplementation protected against hyperglycemia damage by reducing CAT activity and increasing GPx and SOD activities, although it had no influence on MDA levels. Interestingly, only diabetic rats restored myosin-IIB expression at both transcriptional and protein levels in brain tissues after supplementation, and no differences were observed in myosin-Va expression in comparison to non-diabetic rat brains. The functional alterations of antioxidant enzymes and myosins IIB and Va in rat brains of experimentallyinduced diabetes were strongly associated with the disease and aging during acute and chronic stages, but only myosin-IIB expression could be reversed by antioxidants. These data highlight the supplementation (calcium, zinc and vitamin E) antioxidants effects on the oxidative stress and the involvement of myosins IIB and Va in the diabetes stages; however, their mechanisms of action on enzymes activities, lipid peroxidation and myosins expression remain to be elucidated.eng
dc.publisher.countryBRpor
dc.publisher.programPrograma de Pós-graduação em Genética e Bioquímicapor
dc.subject.cnpqCNPQ::CIENCIAS BIOLOGICAS::GENETICApor
dc.publisher.departmentCiências Biológicaspor
dc.publisher.initialsUFUpor
dc.orcid.putcode81763268-
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