Detecção molecular do herpesvírus canino tipo-1 em cadelas com histórico de desordens reprodutivas no Sudeste do Brasil

Abstract

Canine herpesvirus-1 (CaHV-1) is known to cause reproductive disorders and fatal infections in puppies, and may also be found in asymptomatic dogs or related to vaginal vesicular lesions, ocular infections and respiratory diseases. The aim of this study was investigate the ocurrence of CaHV-1 using the polymerase chain reaction (PCR) within urine samples, ocular and vaginal swab samples from Uberlândia, MG, Brazil. Twenty mature bitches, with at least one estrous and reproductive disorders history and/or symptoms were used. Two out of twenty dogs (10%) were positive in the urine and ocular swab samples, respectively, one with pyometra diagnosis and another one healthy with history of stillbirth. No positive results were detected within vaginal swab samples. Sequence analysis of the DNA polymerase gene of the positive samples indicated 100% identity with the sequence of the four CaHV-1 strains selected from Genbank. These findings suggest that positive dogs might be CaHV-1 latent carriers. Additionally, the results confirm the presence of CaHV-1 circulating within urban canine population of Uberlândia, MG. This is the first report that a CaHV-1 infection has been detected in the Southeast Brazil.