Ação da doxiciclina e Artemisia annua L. em cultura de células DH82 infectadas com Ehrlichia canis: estudo ultraestrutural

Abstract

The goal of this job was to evaluate the effect of the infusion of Artemisia annua L. plant in culture of Ehrlichia canis in DH82 cells, analyzing the ultra-structural alterations of this treatment against the canonical treatment with Doxycycline, seeking an efficient alternative for animals that cannot be treated with tetracycline and its products, furthermore to present more affordable to the animal owners. In this experiment it was utilized cultures of DH82 cells infected with Ehrlichia canis, evaluated two and twenty four hours after addition of analyzed substance, distributed as follows: Control group, Doxycycline group and Artemisia annua L. group. After treatments the cell cultures were removed from the bottles by means of "trypsinization" and the pellets produced by the centrifugation process, prepared to ultrastructural evaluation by transmission electron microscopy. The evaluation of the material shows that the Artemisia annua L. has anti-ehrlichiose activity; however, the Doxycycline has shown more efficiency than that one, while both drugs showed to be slightly toxic for DH82 cells.