Isolamento, quantificação e classificação de folículos pré-antrais de suínos

Abstract

The aim of this study was verify the possibility of isolate of porcine preantral follicles by mechanical procedure, in order to obtain an expressive number of viable follicles. Porcine prepubertal female ovaries (n=20) were longitudinally seccionated in two parts. One half was used for simple mechanical procedure and the other half for histological procedure. The average of recovered follicles per ovary by mechanical procedure was 599,160 ranging from 30,400 and 1,200,800 with a direct relationship (P<0.05) between ovarian weight and recovered follicles. In relation of quality 76.44% were viable after mechanical isolation. The presence of granulosa cells around the oocyte 35.67% and 60.27% were denuded and partially covered with granulosa cells and 4.06% was totally covered. The preantral follicles estimated in situ was 131,937,78 ranging on 67,599,04 and 291,897,51. The population distribution, diameter and number of granulosa cells, observed in situ, were 89.41%, 33.04&#956;m and 5.88 on primordial follicles, 2.61%, 47.59&#956;m and 16.45 on primary follicles and 7.98%, 79.11&#956;m and 46,27 on secondary follicles. In conclusion, the mechanical procedure can be used with success on isolation of viable preantral follicles of porcine prepubertal females.