Enxerto osteocondral alógeno, associado à inoculação de células mononucleares da medula óssea e proteína morfogenética óssea no reparo do sulco troclear de coelhos

Abstract

Twenty-four adult, male New Zealand rabbits were separated in two groups of equal number (GI control and GII trated), and subdivided in GI-45, GI-90, GII- 45 E GII-90. The animals of GI and GII were submitted to the osteochondral allograft of the trochlear groove preserved in 98% glycerin. The treated group received an intra-articular injection with 2 x 106 mononuclear cells and 1 μg of recombinant human bone morphogenetic protein-2 (rhBMP-2). The cells isolation went through with ficoll-paque gradient density, with the mean yield of 6,25 x 106 cells/mL and 93,56% cellular viability. Was displayed in both groups periarticular osteophytes and white tissue covered the implant. The graft osteointegration was seen in ten rabbits of GI (83,33%) and three of GII (33,33%). The GII-45 rabbits showed better results in the formation of hyaline cartilage between the implant and the donor site, however, with irregular shape. The inoculation of bone marrow mononuclear cells of rabbits associated with rhBMP-2 promote the osteointegration of osteochondral allograft, however, the regeneration of cartilage was incompletely.