Ultraestrutura de células parasitadas por Ehrlichia spp., métodos diagnósticos e histopatologia em órgãos de cães com erliquiose da micro-região de Uberlândia-MG

Abstract

The objective of this study was the comparison of the diagnostic between peripheral blood smears, venous blood and leuconcentration, and nested-PCR as diagnosis methods for canine erlichiosis. Also was objective to study the histopathological alterations of organs from animals that died, as well as the ultrastructural characteristics of Ehrlichia spp. and Anaplasma platys and confirm the cytological diagnosis of Babesia spp. utilizing seminest-PCR . In order to do this, were selected 56 dogs with morule presence and/or atypical granulation in leucocytes and/or morule presence in platelets of peripheral blood smears, suggestive of infection by Ehrlichia spp. or Anaplasma platys, respectively. Two dogs that have died were utilized to histopathological and ultrastructural studies of organs. Six dogs that have presented merozoites in light microscopy were utilized to confirm the Babesia spp., diagnosis. We concluded that the use of cytology for detection of intracytoplasmatic inclusions of peripheral blood stained with Giemsa is a significant diagnosis method in infections caused by Ehrlichia spp. and Babesia spp.; but for Anaplasma platys it was less sensitive when compared with nPCR. Peripheral blood smears of external ear margin stained with Giemsa was found the more sensitive cytological technique when compared to other kind of extensions. Nested PCR was the less subjective method for CME diagnosis.