Validação de métodos para teste de germinação em sementes de espécies florestais da família Fabaceae

Abstract

CHAPTER II: The standardization of inter-laboratory results of germination test of forest species seeds requires that the methods be robust and subject to low variability. Therefore, the objective was to compare and discuss different forms of the coefficient of variation for normal seedlings in the method validation process for testing germination of seeds of 20 species of the Fabaceae family. Coefficients of variation for the experiment by lot and by laboratory were calculated for normal seedlings from the statistical analysis of method validations. For normal seedlings of 20 Brazilian forest species, the coefficients of variation were low (up to 9.84%), to average (up to 17.66%), contrary to expectations due to high genetic variability in these barely improved species. The increase of the coefficient is not related to treatment for breaking dormancy, but it grows as the lot quality decreases. The high coefficients by laboratory, overestimated by the lot effect, are uniform indicating that the methods are repeatable. The coefficient is not an indicator capable of predicting the heterogeneity of model variance. As normal distribution models random events, randomness is present in the validation process of the 20 forest species of the Fabaceae family. CHAPTER III: There are many treatments to overcome dormancy. However, descriptions of the consequences of these methods in seedling development are scarce. Because of the relevance of Fabaceae family in the context of dormancy seeds, seedlings and seeds of 10 forest species were evaluated quantitatively and qualitatively as to damage and infections caused by invasive treatments. Scarification, cutting and preheat methods were applied to seeds. They were then sampled was arranged in germitest, forming rolls distributed in a germination boxes under continuous fluorescent white light at 25 °C. Root protrusion used as the sole criterion, overestimates the efficiency of germination treatments to overcome dormancy. Scarification and cutting are efficient for Enterolobium contortisiliquum, Parkia pendula, Senna multijuga and Senna macranthera seeds, as well as cutting for Mimosa caesalpiniaefolia seeds. However, they are inefficient for Dimorphandra mollis and Enterolobium maximum because of the increase in the percentage of infected seedlings, and to Stryphnodendron adstringens, for dead seeds. The high percentage of damaged seedlings of Erythrina velutina and Erythrina speciosa cannot be attributed solely to treatment, because without pretreatment the root system gets strapped, forming a loop. The preheat treatment is inefficient because it results in a high percentage of hard seeds remaining.