Desenvolvimento de imunoensaio para confirmação da infecção de Mogiana Tick Virus (MGTV) em bovinos em fazendas da região do Triângulo Mineiro

Abstract

In 2011, a virus isolated from cattle infested with Rhipicephalus microplus ticks in Brazil, named Mogiana tick virus (MGTV), was identified. In 2014, MGTV was associated with the Jingmenvirus group, a subdivision of Flavivirus discovered in China. Ticks, members of the Ixodidae family, are the main vectors of these viruses, being considered the second-largest transmitters of diseases caused by pathogens by the World Health Organization (WHO). In addition to affecting humans, ticks cause significant damage to cattle farming in Brazil, with estimates of direct and indirect losses amounting to around 3.2 billion dollars annually, making it essential to understand sanitary aspects related to this parasitism. This proposal involves the use of the Enzyme Linked Immuno Sorbent Assay (ELISA) test, with the viral protein 2 of MGTV as an antigen, aiming for rapid and accurate diagnosis in bovine sera. The effectiveness of this method is subject to confirmation by western blotting, a protein transfer technique for detecting antigen-antibody binding.