1. Repositório Institucional - Universidade Federal de Uberlândia
2. Instituto de Ciências Biomédicas (ICBIM)
3. TCC - Ciências Biomédicas

ORCID:	http://orcid.org/0009-0009-3244-4785
Document type:	Trabalho de Conclusão de Curso
Access type:	Acesso Aberto
Title:	In vitro assessment of antitumor and antiparasitic effects of extracts and fractions from Persea Americana and Syzygium cumini
Alternate title (s):	Avaliação in vitro dos efeitos antitumorais e antiparasitários de extratos e frações de Persea Americana e Syzygium cumini
Author:	Baldo, Luana Brasil
First Advisor:	Santiago, Fernanda Maria
First member of the Committee:	Santiago, Fernanda Maria
Second member of the Committee:	Torres, Jhoan David Aguillón
Third member of the Committee:	Justino, Allisson Benatti
Summary:	Persea americana (Avocado) of the Lauraceae family and Syzygium cumini (Jambolan) of the Myrtaceae family are food and medicinal plants widely used with antioxidant, anti-inflammatory, antitumor, and antiparasitic activities. Objective: The present study investigated in vitro the antitumor and antiparasitic effects of extracts and fractions purified from P. americana and S. cumini. Materials and Methods: Fractions were obtained from the crude ethanolic extract of P. americana seed (EtOHPaS), P. americana fruit peel (EtOHPaP) and Syzygium cumini leaves (EtOHScL) using polar solvents: hexane (fractions FHPA and EHSc), dichloromethane (fractions FDMPA and EDMSc), ethyl acetate (fraction FAePA), nbutanol (fractions FnBPA and EnBSc) and water (fractions FaqPA and EAqSc). Immunohemolysis and MTT assays were performed against the HFF, Beas-2B and Pnt-2 nontumor cells to assess the cytotoxicity of the samples. To evaluate the antitumor activity, MTT assays were performed using PC3 (prostate cancer cell) and A549 (lung cancer cell). Colony assays were also performed to evaluate the inhibitory effect of the samples on colony formation. Regarding the antiparasitic activity, plaque assays were performed to analyze the effect of the samples on the invasion and proliferation process of Toxoplasma gondii tachyzoites. Results: The results showed that the FAePA fraction of EtOHPaS exhibited high antitumor activity at a concentration of 400 μg/ml, with a A549 tumor cell death percentage of 45%. The EtOHScL and the EHSc and EDMSc fractions had higher antiproliferative effects against PC3 tumor cell. Furthermore, EtOHPaP and its FHPA fraction; the FAePA, FDMPA, FaqPA fractions of EtOHPaS; and EtOHScL and its EnBSc, EAqSc, EDMSc, and EHSc fractions demonstrated antiparasitic properties indicating that these treatments effectively inhibited the proliferation and reinvasion of T. gondii tachyzoites. Conclusions: In that sense, the fractions obtained from EtOHPaP, EtOHPaS and EtOHScL show promising therapeutic potential. More studies are required to investigate the mechanisms which may be responsible for the biological activities of these plants.
Keywords:	Syzygium cumini; Persea americana; tumor cells; Toxoplasma gondii.
Area (s) of CNPq:	CNPQ::CIENCIAS BIOLOGICAS::PARASITOLOGIA CNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIA::IMUNOLOGIA CELULAR
Language:	eng
Country:	Brasil
Publisher:	Universidade Federal de Uberlândia
Quote:	BALDO, Luana Brasil. In vitro assessment of antitumor and antiparasitic effects of extracts and fractions from Persea Americana and Syzygium cumini. 2024. 26 f. Trabalho de Conclusão de Curso (Graduação em Biomedicina) – Universidade Federal de Uberlândia, Uberlândia, 2024.
URI:	https://repositorio.ufu.br/handle/123456789/43928
Date of defense:	21-Nov-2024
Appears in Collections:	TCC - Ciências Biomédicas

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