Bactérias diazotróficas produtoras de biofilme isoladas da filosfera de plantas infestantes em ambientes de Cerrado.

Abstract

The phyllosphere, the surface of the leaves, houses a great diversity of microorganisms, especially in native forests, such as the Cerrado. These microorganisms can help promote plant growth in several ways, such as fixing atmospheric nitrogen and producing biofilm. In this study, five plants of two different species were collected in areas with less human and agricultural interference. The microorganisms present in the leaves of the collected plants were isolated through serial dilution and microbial growth in different culture media and subsequently characterized regarding the appearance of the colonies, shape, arrangement and gram color. In addition, biofilm production was analyzed using the crystal violet method and DNA extraction, PCR and gel electrophoresis visualization procedures to detect the nifH gene in the samples. The results showed that the isolated bacteria are good biofilm formers in general and that one of the samples has the nifH gene in its genome, which reveals promising performance for possible agricultural applications, with the potential to stimulate plant growth.