1. Repositório Institucional - Universidade Federal de Uberlândia
2. Instituto de Ciências Biomédicas (ICBIM)
3. TCC - Ciências Biomédicas

ORCID:	http://orcid.org/0009-0004-8997-4131
Document type:	Trabalho de Conclusão de Curso
Access type:	Acesso Aberto
Title:	Análise de parâmetros hematológicos e sua relação com alterações histopatológicas em modelo murino de carcinogênese lingual induzida por 4-nitroquinolina-N-óxido e etanol
Alternate title (s):	Analysis of hematological parameters and their relationship with histopathological alterations in a murine model of tongue carcinogenesis induced by 4 nitroquinoline-1-oxide and ethanol.
Author:	Pereira, Isabella Moura
First Advisor:	Faria, Paulo Rogério de
First coorientator:	Costa, Anaíra Ribeiro Guedes Fonseca
First member of the Committee:	Cardoso, Sérgio Vitorino
Second member of the Committee:	Hiraki, Karen Renata Nakamura
Summary:	Peripheral blood biomarkers are significant prognostic predictors for several human tumors, as they reflect the dynamics between anti-tumor and tumor-promoting effects of the inflammatory response. Studies have shown that increased levels of neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and systemic immune-inflammation index (SII) are associated with unfavorable outcomes for oral squamous cell carcinoma (OSCC). This study aimed to analyze the association of tumor inflammation-related hematological alterations to the incidence of epithelial dysplasia (ED) and OSCC in mice treated with tobacco mimicker 4-nitroquinoline-N-oxide (4NQO) and ethanol. Methods: 120 C57Bl/6J mice equally divided into males and females were randomly allocated to four groups (n=15): PPG/H2O, treated with vehicle propylene glycol (PPG) for 10 weeks followed by drinking water (H2O) for 15 weeks; PPG/EtOH, treated with PPG for 10 weeks and 8% ethanol (EtOH) for 15 weeks; 4NQO/H2O, treated with 100ug/mL of 4NQO diluted in PPG followed by H2O for 15 weeks; and 4NQO/EtOH, treated with 4NQO for 10 weeks followed by EtOH for 15 weeks (CEUA-UFU, #020/21). At the end of the 25-week treatment period, animals were euthanized and necropsied. Tongues were submitted to histopathological analyses for ED and OSCC diagnoses. Blood samples were collected and processed for red blood cells, white blood cells, and platelet cell parameters analyses. The NLR, PLR, and SII were also calculated. Results: Nearly 93% of the males from the 4NQO/H2O and 4NQO/EtOH groups developed OSCC. In females, OSCC was observed in 66.7% and 60% of the 4NQO/H2O and 4NQO/EtOH groups, respectively. This difference between males and females from the 4NQO/EtOH group was significant (p=0.04). Neutrophil count, NLR, and SII increased significantly from the control group to the 4NQO/H2O and 4NQO/EtOH groups containing OSCC-affected male and female mice, while lymphocyte count decreased. Females of the 4NQO/H2O group with ED also had significantly higher neutrophil, NRL, and SII values than the normal mucosa. Conclusion: White blood cell counts and NRL changed significantly during epithelial tumorigenesis, reflecting tumor inflammation-related systemic alterations that could be further explored as biomarkers of OSCC development and progression.
Keywords:	Carcinogenesis Mice Hematological tests Oral cancer Tumor biomarkers
Area (s) of CNPq:	CNPQ::CIENCIAS DA SAUDE
Language:	eng
Country:	Brasil
Publisher:	Universidade Federal de Uberlândia
Quote:	PEREIRA, Isabella Moura. Analysis of hematological parameters and their relationship with histopathological alterations in a murine model of tongue carcinogenesis induced by 4 nitroquinoline-1-oxide and ethanol. 2024. 30 f. Trabalho de Conclusão de Curso (Graduação em Biomedicina) - Universidade Federal de Uberlândia, Uberlândia, 2024.
URI:	https://repositorio.ufu.br/handle/123456789/42011
Date of defense:	30-Apr-2024
Appears in Collections:	TCC - Ciências Biomédicas

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