Efeito dos metabólitos e das vesículas extracelulares de bactérias probióticas do gênero bacillus no controle de Salmonella Heidelberg

Abstract

Poultry is one of the major sectors of Brazilian agribusiness. Concern for the health of animals and the quality of their products is a reality in the country. Several pathogens are responsible for the diseases found in the field and offer risks to public health, since they can be transmitted via food to man. The biggest challenge is to count them safely, without generating resistance to antimicrobials, by excessive use of antibiotics. For this reason, other alternatives such as probiotics, which are food additives, can be used. In this work we evaluated the inhibitory effect of probiotics Bacillus Subtilis, Bacillus Cereus and Bacillus Licheniformis, their metabolites and extracellular vesicles produced by these probiotic strains, when exposed to the pathogenic strain of Salmonella Heidelberg. In this study, the samples were tested in different ways in solid medium and liquid medium in order to verify the formation of inhibitory halos and quantify proteins. The metabolites in solid medium did not have much effect on the inhibition of bacteria, but in liquid medium were able to inhibit it. The vesicula of Bacillus Licheniformis, in turn, had a small effect under Salmonella, demonstrating that these probiotic fragments can be exploited in the control of pathogenic bacteria.