Efeito de dois diferentes tipos de própolis brasileiras sobre bactérias e células pulpares humanas

Abstract

Brazilian propolis has gained notoriety due to its benefits and pharmacological effects. At the same time, the adoption of conservative endodontic techniques such as pulp therapy has been prioritized in minimally invasive dentistry. In this scenario, the present study evaluated the impact of direct application of hydroalcoholic extracts of Brazilian green and brown propolis, BGP and BBP respectively, on human pulp cells and endodontic pathogens. Both propolis, have shown positive results in other cell lines, but little evaluated on pulp tissue. After 24h of cell planting, the cells were kept in direct application and contact for 24h of the materials: BGP (5, 10 and 50 μg/mL), BBP (5, 10 and 50 μg/mL), DMEM (control group), TNF-α 10μg/mL and DMSO 0.5%, Carbamide Peroxide 0.018% (CP) contact for 30min. After period of contact, cell viability (MTT), quantification of reactive oxygen species (ROS, DCFH-DA probe), nitric oxide (NO, Griess reagent) and cell morphology (SEM) were evaluated. The pH measurement occurred before and after 24 hours of contact with the cells. The cell migration test (Wound heal) compared the immediate contact and after 24h and 48h of the application of the materials. In addition, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the two propolis were performed against a panel of new bacteria involved in primary endodontic infection. Data analysis used in the One and Two-Way ANOVA complemented by Tukey (α= 5%). Cell viability and NO production showed no statistical difference between BGP, BBP and DMEM groups (p<0.05). In the production of ROS, there was difference between TNF-α and the other groups. The CP group had a lower mean initial pH (7.90, DV=0.7). Regarding the antibacterial effect at concentrations ≤100 μg/mL, BBP showed for seven bacteria while BGP only for P. micra. It was concluded that BGP and BBP showed high antibacterial activity, high cytocompatibility, absence of oxidative stress production and BGP showed greater stimulation of cell migration in human dental pulp cells.