Determinação da fibra em detergente ácido em diversos alimentos pela técnica da autoclave utilizando saco filtrante e cadinho filtrante

Abstract

The original method for acid detergent fiber (ADF) analysis requires individual labor for each evaluated sample, which takes a long time to analyze a large quantity of samples. Alternative methods have been developed to make the analysis more practical, reduce the time and labor involved, including the use of an autoclave instead of the reflux equipment in the original method, and the use of filter bags instead of the filter crucible. Thus, the objective of this study is to evaluate the ADF analysis using the autoclave method with a filter bag made of non-woven fabric (TNT) weighing 100g/m², and the filter crucible, in order to determine if there are differences between the use of these materials in the ADF results. The samples used were total diet and concentrate for cattle, Tifton hay, sorghum silage, corn, and soybean meal, totaling six feedstuffs. The experimental design used was completely randomized, with two treatments (filter crucible and filter bag) and six replicates for each evaluated feedstuff. The obtained results were subjected to analysis of variance, and the means were compared using the F-test at a 5% probability level, using the statistical software R version 4.4.2 (2022). Statistical differences were observed for all feedstuffs between the techniques of using the filter bag and the filter crucible, with higher ADF values obtained when using the filter bags. The ADF analysis using the autoclave and the non-woven fabric filter bag weighing 100g/m² yields superior results compared to the use of the filter crucible.