Extratos solúveis de Epimastigotas de Trypanosoma Cruzi modulam a migração, proliferação e multiplicação de células tumorais de mama triplo negativas

Abstract

Triple-negative carcinomas are known to have an aggressive tumor profile, with high rates of sameness and a difficult prognosis. In the search for antitumor elements, surprisingly, microorganisms including Trypanosoma cruzi and some of its functions play antiproliferative and antimetastatic activities in breast tumor cells, such as the P21 protein. Therefore, this work aimed to analyze the effects of soluble extracts (ES) of epimastigote forms of T. cruzi, being wild (WT), knockouts for the P21 gene (P21/KO), and control of the silencing technique (Cas9), in the chemotaxis and negative cell responses of triple breast cancer cells. Epimastigotes were lysed by freeze/thaw collecting ES, which was evaluated for its protein profile by Bradford's method and electrophoretic separation technique. As for cancer cells, cell viability tests were performed with treatments by ES in biochemical assays with Resazurin; cell chemotaxis assays by Transwell when stimulated by ES; colony formation assays, and mitosis analysis by immunofluorescence after treatments with soluble extracts. Statistics tests were applied, and the significance of the data occurred when p <0.05. It was observed that the different band profiles, possibly like each other; treatment with ES maintained as viable cells, in addition to mediating directing migration, colony formation, and mitosis of MDA-MB-231; there were different results between the WT and P21/KO groups, note that the absence of P21 positively modulates clonogenic and mitotic processes. Therefore, the ability of extracts to phenotypically promote tumor progression should be avoided, in addition to noting the potential of P21 in mediating antitumor responses. Furthermore, this work supports future studies, at the biomolecular level, to elucidate the mechanism of action of soluble extracts of epimastigotes on tumor cells.