Produção de anticorpos policlonais anti - Erysipelothrix sp. Strain 2

Abstract

The pathogenic microorganisms are a major defiance faced by the poultry industry today. The Erysipelothirx spp genus are known nowadays for being an occupational zoonosis, also for causing outbreaks in farm animals, including the poultry industry. The recent finding of a new species, Erysipelothrix sp strain 2, requires further studies to be carried out to comprehend more about its pathogenicity, diagnose, control and prevention. The objective of the present study was to produce polyclonal antibodies anti - Erysipelothrix sp strain 2, which may be used in further studies about these bacteria. To obtain the antibodies was used two female rabbits, which was allocated in the ixodology laboratory of Universidade Federal de Uberlândia through the entire procedure of immunization. The collection was performed pre and post immunization of both rabbits so that serum aliquots were prepared and analyzed. The antibodies characterization and quantification were performed using an ELISA test which showed that immunized animals had high anti Erysipelothrix sp strain 2 titration. From that study, further research may be driven to develop a better diagnose test and better understanding of the host-pathogen relationship of a species that is still poorly studied.