Comparação de dois materiais biocerâmicos no manchamento após pulpotomia, viabilidade e estresse oxidativo de células pulpares: estudo in vitro

Abstract

This study aimed to compare tooth discoloaration in immature teeth mimicking total and partial pulpotomy using Biodentine (BD) and Bio-C repair (BCR) and evaluated the cytotoxicity of the materials to pulp cells (hDPCs). Seventy-two bovine mandibular incisors were prepared to simulate complicated coronary fractures in an immature tooth. In the pulp tissue simulation, the roots were filled with a mixture of agar and blood (control group), and BD and BCR were placed on it with approximately 2 mm of thickness. The color evaluation analyzes of the samples were performed before and immediately after the pulpotomy and repeated at 30, 60 and 90 days. The hDPCs were contacted with different dilutions of culture media previously exposed to such materials and tested for cell viability using the MTT assay. Subsequently, hDPCs were stimulated through contact with lipopolysaccharides (LPS) and exposed to extracts, for quantitative measurement of reactive oxygen species (ROS) and nitric oxide (NO). Data were evaluated by ANOVA followed by the Tukey and Dunnet test (α = 0.05).For tooth descoloration, there were significant differences for “pulpotomy material” (P<0.001) and “evaluation time” (P<0.001) and for the interaction “material*evaluation time” (P=0.004). In the cell viability analysis, BD and BCR were similar to each other in all evaluated dilutions and similar to the control (P=0.3660). BCR showed lower production of ROS and ON in relation to the LPS group (C+) (P<0.05) and similar to DMEM (C-) (P>0.05). BD showed ROS and NO values similar to the two control groups (P>0.05). Both BCR and BD when used in total or partial pulpotomy can significantly change the color of teeth to a perceptible degree. Although both materials are biocompatible, BCR decreased the production of ROS and NO in hDPCs stimulated with LPS.