Associação da laserterapia em baixa intensidade e das proteínas derivadas da matriz do esmalte com biomateriais osteocondutores: Estudo pré-clínico em ratos

Abstract

The aim of this study was to evaluate whether the association of low-level laser therapy with infrared laser (LLLT) and enamel matrix-derived proteins (EMD) improves the repair of grafted areas with deproteinized bovine bone (DBB). For this study, 24 rats were used. These animals were randomly allocated into 2 groups with 12 animals each, which were evaluated in two experimental periods (30 and 90 days), with 6 animals in each group and experimental period. The groups were divided according to the type of treatment association applied to the grafted areas: Group 1: DBB-filled capsule that was associated with enamel matrix-derived protein (EMD); Group 2: DBB-filled capsule associated with EMD + LLLT. A Teflon capsule in the form of a dome was installed bilaterally in the ramus of the mandible of each animal, which was filled with DBB that was later fixed in the mandible by means of sutures with 4.0 silk thread that passed through the capsule itself and the perforations performed in the ramus. of the jaw. Microtomographic analyzes were performed to evaluate the volume of mineralized tissue. It was verified that the areas grafted and treated with EMD associated with LLLT presented greater amounts of mineralized tissues than the group where the areas grafted with OBD were treated only with EMD in both experimental periods. Laser therapy associated with EMD promotes greater volume of mineralized tissues than the use of EMD alone in areas grafted with OBD.