Genossensor eletroquímico para detecção de Hantavírus baseado em eletrodos de carbono impresso com nanopartículas de cobre

Abstract

The objective of this study describes the development of an electrochemical genosensor in the construction of carbon ink-based printed electrodes (PCE), with electrodeposition of copper nanoparticles for indirect detection of methylene blue peak current on Hant 2 target. Genosensors are classified as DNA or RNA electrochemical biosensors, they are devices that have advantages such as high sensitivity, selectivity and short-response time. The Hantavirus infection is caused by inhalation of aerosols from the saliva, urine and feces of specific and infected rodent populations. In the regions of Asia and Europe, called regions of the Old World, there is a lethality rate is up to 15%, causing Hemorrhagic Fever with Renal Syndrome (HFRS). At the same time, in the regions of the New World (Americas), responsible for causing infections in the countries of the American continent, including Brazil with a fatality rate of 60%, causing Hantavirus Cardiopulmonary Syndrome (HCPS). The diagnostic methods for Hantavirus, such as ELISA, PCR, IFA, are based on high-cost methods with trained and qualified handlers for the development of work in laboratories with a specific safety level. The work developed evaluated the electrochemical response of the Hant 2 target on the PCE|CuNps platform, highlighting the electronic transfer and an increase in the surface area when compared to PCE without modifications with CuNps. The detection of the virus occurred from the immobilization of the Hant 1 probe on the PCE|CuNps platform using the methylene blue (MB) hybridization indicator and the differential pulse voltammetry technique. From the construction of the analytical curve, the detection limit of 4.56 pg/mL and the quantification limit of 15.22 pg/mL in the stability period of 7, 15, 21 and 30 days. Considering the standard deviation, stability is maintained at 100% in 30 days. Therefore, the developed platform showed fast, effective and low-cost detection, with low detection and quantification limits, being promising for use in point of care.