Vitrificação de tecido ovariano equino nas diferentes concentrações de resveratrol: 10 µM E 20 µM

Abstract

The present study was carried out to compare the different antioxidant solutions in vitrification of equine ovarian tissue, a faster cryopreservation technique compared to slow freezing, evaluating the morphology and viability of vitrified follicles included in the equine ovarian tissue. For that, ovary fragments measuring 3mm x 3mm x 1mm were vitrified on French vane, using two different solutions of vitrification (SV), containing 10 um of resveratrol and 20 um of resveratrol. After 15 days, the fragments of the ovarian tissue were heated and processed for histology for morphological analysis of the follicles. The histological analysis showed that there was no statistical difference between the treatments with SV1Res10 and SV1Res20 in the percentage of morphologically normal follicles after vitrification, with a considerable cytoplasmic retraction of the follicles, and that the control solution was better. In conclusion, vitrification containing resveratrol as an antioxidant for both 10æm and 20æm of resveratrol, so that the time of exposure to the antioxidant agent and temperature, are not most suitable for vitrification of equine ovarian tissue.