Avaliação biológica de arcabouços nanofibrosos magnéticos de pla-peg-óxido de ferro visando desenvolvimento de substituto dérmico

Abstract

The skin acts as a protection against biomechanical stimuli, maintains homeostasis and is responsible for thermoregulation. When injured, the body is able to close the wound, but if the injury is large, the healing is impaired, and the body is exposed to infections. Tissue Engineering aims to develop living structures that could restore damaged skin. In addition to this application, this area has been explored for the production of dermal substitutes used in toxicological and efficacy tests of pharmaceuticals and cosmetics. The so-called triad of Tissue Engineering is composed of cells, scaffolds and stimuli, and the choice of the scaffold material has an important role because it influences the formation of the tissue. The objective of this work was to evaluate the cellular growth of skin cells in a matrix of fibrous polymeric blends, testing its biocompatibility and capacity of proliferation of fibroblasts to build a national skin substitute in vitro. The associated colorimetry and immunofluorescence assays showed that the cells remained intact, without any morphological alteration, grew in depth and were able to produce endogenous extracellular matrix. In addition, the results on cell viability showed that the material is not cytotoxic. Therefore, it could be concluded that the scaffolds used in this work could be considered promising for the development of a dermal replacement.