Avaliação da microtomografia computadorizada do reparo ósseo alveolar: Qual região você deve analisar ?

Abstract

Objeticve: Evaluate the influence of the extraction socket (distal or lingual root) and the type of region of interest (ROI) definition (manual or predefined) on the assessment of alveolar repair after tooth extraction using micro-computed tomography (micro-CT). Materials and Methods: The sample consisted of micro-CT volumes of the mandibles submitted to extraction of the mandibular right first molar (M1) of seven Wistar rats. The reconstructed images were analyzed using the extraction sockets: D- distal and IL- intermediate lingual root; and the ROI: MA- manual, CR- central round and PR- peripheral round. The BV/TV values obtained were analyzed by ANOVA two-way with Tukey post hoc test (α = 5%). Results: In relation to the analyzed extraction socket, the D socket resulted in significantly lower BV/TV values than the IL socket, for the groups MA (P = 0.001), CR (P <0.001), PR (P <0.001). For the region of interest (ROI), BV/TV was significantly higher (P <0.001) for the MA group (75.11 ± 6.69) compared to CR (65.31 ± 5.16) and PR groups, with lower BV/TV for CR (55.96 ± 7.35) in the D socket. However, no significant difference was observed for the groups MA (91.38 ± 4.32, P = 0.855), CR (92.12 ± 5.49, P= 0.769) and PR (93.08 ± 4.16, P = 0.453) in the IL extraction socket. 4 Conclusion: Different ROIs definitions and the extraction socket being analyzed affect the morphometric results in micro-CT. The predefined method with standardized ROI in the central region of the bone defect in the distal extraction socket resulted in a more effective assessment of bone volume demonstrating the most critical region of the bone neoformation process.