Microtomografia computadorizada: comparação de parâmetros utilizados na aquisição, reconstrução e processamento de imagens no estudo do processo de reparo ósseo e aplicabilidade na análise do efeito de diferentes terapias

Abstract

The use of microcomputed tomography (microCT) has largely increased. Issues related to its application and parameter definitions still need to be investigated. The objective 1 of this work was to conduct a literature review on the use of microCT in Dentistry for bone tissue investigations and the steps involved in the methodology. The literature review indicated that the characteristics of the sample and the parameters to be evaluated should be considered so that the process of image acquisition, reconstruction and processing are properly carried out. The second objective was to investigate the influence of variables in the microtomographic analysis of bone repair, such as: voxel size and filter thickness used in image acquisition, and the experience of examiners in data analysis. Objective 3 was to determine whether image binning and frame averaging during microCT acquisition affect the morphometric results of bone tissue repair. Objectives 2 and 3 demonstrated that voxel size and image binning affect data analysis, demonstrated by the differences observed in the Tb.Th and BV/TV parameters. Finally, in objective 4, the application of microCT, in association with other methodologies, was used to assess the effect of radiotherapy and lowintensity laser therapy (LLLT) on bone repair of defects grafted with deproteinized mineralized bovine bone (DBBM) and defects filled by clot. It could be concluded, that the amount of neoformed bone tissue observed was smaller in the groups submitted to radiotherapy, and no difference was observed in the results in LLLT groups. The incorporation of graft particles with the newly formed bone was preserved, demonstrating that the osteoconductivity of the biomaterial was maintained even after radiotherapy.