Níveis de IgA l e expressão de ácido ribonucléico mensageiro para interferon gama e fator de necrose tumoral alfa, em saliva total de pacientes com diabetes mellitus tipo II portadores de doença periodontal crônica

Abstract

The periodontal disease has been characterized as an oral infection that results in gingival inflammaíion, desíruction of periodontal tissues, alveolar bone loss and sometimes lost of the teeth in severe cases. Diabetes mellitus consists in a group of disorders that include reduction or absence of insulin. The relationship between diabetes mellitus and periodontal disease is well discussed. Studies has been shown that diabetic patients type íí have higher leveis of serum IgA (IgAl seems contribute for this event) and alteration in the cytokine production. The severity of periodontal disease could suggest some salivary differences in the IgAl titers and in the cytokine expression In this work was tested the hypothesis that the diabetic patients type II with moderate periodontal chronic disease (MPCD) could present difference between IgAl salivaiy titers and cytokine expression when compared with the severe periodontal chronic disease (SPCD) cases. It was employed the reactivity to Jacalin to determine IgAl titers by ELISA, and RT-PCR to detect the cytokines expression in saliva of 13 diabetic patients with MPCD and 11 with SPD. The results showed that the titers o f IgAl in MPCD were higher than SPCD, with a higher prevalence of the IgA 1 titer 64 in SPCD and IgAl titer 512 in MPCD. Furthermore, was showed that IFN-y mRNA was highly expressed in SPCD group than MPCD group and the expression of the TNF-a mRNA was similar in both groups. On the other hand, IL-13 mRNA and IL-4 mRNA did not detected in any saliva samples of the analyzed groups. Therefore the results of present work suggest that IgAl could have a partial protection in MPCD group and mRNA expression for TNF-a and was not correlate to disease severity degree