Modulação das citocinas IL-6 e TGF-B após o tratamento de células de câncer de próstata com 4-nerolidilcatecol

Abstract

Prostate cancer (PCa) is a public health problem worldwide and the fourth most common tumor considering both sexes. Castration-resistant PCa (CRPC) is an advanced stage of PCa that develops even after pharmacological or surgical androgenic block, with a worse prognosis. Natural products (PNs) have been widely studied due to their bioactive properties with antitumor and anti-inflammatory activity. 4-nerolidylcatechol (4-NC) attracts scientific interest because it’s cytotoxic effect on some tumor straisn. The present study aimed to evaluate the immunomodulatory potential of 4-nerolidylcatechol (4-NC), the main secondary metabolite obtained from Photomorphe umbellata, in the PC3 (CRPC) cell line. The MTT assay confirmed the cytotoxicity of 4-NC against the LNCaP and PC3 tumor strains, and the concentration of 50µM was chosen for further experiments. The transcripts of the cytokines IL-1, IL-6, and TGF-β, involved in the inflammatory process of PCa, were quantified by qPCR after treatment of PC3 cells with 4-NC for 48h. The compound modulated the expression of IL-6 and TGF-β, decreasing the transcripts in 40% and 15%, respectively, when compared to the control. However, 4-NC had no effect on IL-1. In addition, the compound also did not change the levels of nitrite in the supernatant of these cells. Therefore, the results demonstrate an immunomodulatory profile of 4-NC in advanced stages of PCa, capable of decreasing the inflammatory process in the tumor microenvironment and the progression of the disease.