Atividade anti-inflamatória do extrato aquoso das folhas da Eugenia dysenterica (cagaita) em modelo experimental de inflamação peritoneal em camundongos BALB/c

Abstract

The Eugenia dysenterica species, known in Brazil as cagaita, is part of the Myrtaceae family. Phytochemical studies demonstrate that this species produces, among the secondary metabolites, flavonoids and tannins, which are the anti-inflammatory activities well used in the literature. Various plant compounds of E. dysenterica have been used in studies that have evaluated their pharmacological properties, among them, anti-inflammatory. However, to date, there are no data available on the anti-inflammatory potential of the aqueous extract of the leaves of the species. Thus, the present research project investigated the anti-inflammatory activity extracted from E. dysenteric leaves in an in vivo experimental model of peritoneal inflammation induced by carrageenan (CGN). BALB/c mice were divided into four groups (G): G1: animals that received orally (v) carboxymethylcellulose (CBX) representing the negative control group for anti-inflammatory action; G 2, 3 and 4: isolated animals with different levels of extraction (100, 500 and 1,000 mg / kg, respectively). Sixty minutes after the v.o. procedures, animals in all groups received intraperitoneal (i.p.) injection of 1% CGN to induce peritoneal inflammation. After four hours, the animals were anesthetized and killed by cervical dislocation. Then, the peritoneal cavity was gently washed with phosphate buffered saline (PBS), pH 7.2, and the peritoneal lavage was collected for total and differential leukocyte counts. The results obtained showed that there was a reduction of total leukocytes for the peritoneal cavity in the animals that received aqueous extract of the leaves of E. dysenterica, in all ranges used. The differential leukocyte count showed a statistically significant reduction in lymphocytes in the peritoneal cavity of animals eaten with extract; however, this effect was not observed with the detection of neutrophils and monocytes. Together, these data suggest a possible anti-inflammatory action of the aqueous extract of the leaves of the cagaita.