Determinação simultânea de hidroclorotiazida e metoprolol por eletroforese capilar com detecção condutométrica sem contato (CE-C4D)

Abstract

In this work, capillary electrophoressis with contactless capacitive coupled conductivity detection technique (CE-C4D) was used for the development of a fast method of quality control of hydrochlorothiazide (HCT) e metoprolol (MET) pharmaceutical formulations. For simultaneous determination, the buffer (BGE) was composed by 10 mmol/L of H3BO3 adjusted with NaOH to pH = 9,0, with analysis time less than 80 seconds. The limits of detection (LOD) were 30 and 65 mol/L for HCT and MET, respectively. Calibration curves showed correlation coefficients greater than 0,993 and RSD values less than 5% (n=10). The metod presented recovery values of 95% and 102% for HCT and MET, respectively. Furthermore, the CE-C4D methods are simple, generates a minimum amount ofwaste (lower environmental impact), are low cost (equipment and maintenance) and has a high analytical frequency: 34 injetions h-1.