Wound Healing Effect Of ScLL Lectin from Synadenium Carinatum Latex in murine model

Abstract

Wound healing is a physiological event that leads to the restoration of normal structure and in damaged tissues. Several natural products, such as lectins, have been shown to accelerate the healing process. Thus, in the present study, the Synadenium carinatum latex lectin (ScLL) was isolated and investigated for its healing properties that optimizes the restoration process of the injured tissue. Dorsal skin wounds were created in mice, which were divided in groups treated with ScLL at concentrations of 250 and 500 μg/ml and group treated with water (control). ImageJ program was used to measure the contraction of wounds and tissue sections were stained with Haematoxilin and Eosin for histological assay using Picro-Sirius Red for collagen determination content. Concerning about the results, ScLL lectin, in the macroscopic evaluation demonstrated better performance in the wound contraction, when compared to control. In the histological analysis, the ScLL presented an intense re-epithelialization level occurring faster than control group. In addition, we showed that ScLL 500 µg/ml presented a lower concentration of collagen than control group, however possesses anti-keloids properties by its ability to reduce exacerbated deposition of collagen.