Alterações moleculares no fígado induzidas pela dieta rica em gordura e nitrato de sódio utilizando espectroscopia de infravermelho com transformada de Fourier

Abstract

Monitoring liver changes is a time consuming and costly practice. Consequently, the search for a faster, specific, low-cost method without laborious protocols to detect obesity-induced liver changes is of great interest. Attenuated Full Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR) has been used in disease monitoring, but tissue applications are incipient. ATR-FTIR spectroscopy was used to evaluate the liver of C57BL / 6 mice with control diet (CD + Placebo), hyperlipid diet (HFD + placebo), control diet + sodium nitrate (CD + NaNO3) and hyperlipid diet + sodium (HFD + NaNO3) to identify possible obesity-related spectral biomarkers. The hepatic spectrum of mice in the studied groups presented fourteen vibrational modes, eight of which were pre-validated as biomarkers for monitoring liver changes by the ROC curve. In addition, NaNO3 treatment promoted reduction in six vibrational modes affected by HFD. In total, the spectral biomarkers 3008, 2923, 2854, 1741, 1459 and 1161 cm-1 may provide a robust new alternative for monitoring liver-induced changes in obesity. In addition, 1229 and 923 cm-1 are spectral candidates to indicate liver damage by NaNO3.