Validação de primers microssatélites para Campomanesia adamantium (CAMBESS.) O. BERG

Abstract

Cerrado is considered the richest savannah on the planet sheltering more than 10,000 species of plants. One of the species present in this biome is Campomanesia adamantium, known as gabirobeira, guavira or gabiroba belongs to the family Myrtaceae. Its fruit with specific flavor and its high content of sugar and vitamin C has great appreciation of the population, and can be consumed in natura, in the form of sweets, ice cream and drinks. Although it presents great economic potential, studies with molecular markers in the C. adamantium species for breeding programs are scarce. In this context, the work was carried out with the objective of validating Eucalyptus sp primers for the amplification of microsatellites in C. adamantium. Four plants were collected in a population of the species in the municipality of Coromandel and the DNA was extracted from young and healthy leaves. PCR was performed with twelve Eucalyptus spp primers at annealing temperatures described by literature and the actual annealing temperature of the primers. Amplified fragments were analyzed from 8 % polyacrylamid gel electrophoresis. The DNA concentration of each sample had a mean of 617.46 ng / μL,. Of the 12 primers, four primers did not present amplification products at the two evaluated temperatures; one did not amplify at annealing temperature of literature and amplified non-specific fragments at the actual annealing temperature; one amplified non-specific fragments at the two annealing temperatures; three amplified specific and non-specific fragments at the two temperatures; and two, in which the temperature described by literature and real primer temperature is similar amplified non-specific fragments.. The present study indicated that there was no transferability of Eucalyptus spp. for C. adamantium, demonstrating the need for new evaluations with microsatellite primers of species closer to C. adamantium.