Transferibilidade de primers microssatélites de miconia calvescens dc. para miconia albicans (sw.) triana (melastomataceae)

Abstract

The genus Miconia, is the largest in the Melastomataceae, one of the largest neotropical genera, with more than 60% of apomic species. Miconia albicans is a shrub, characteristic of the Cerrado, the savannas of Central Brazil, where it is observed in high density in areas of regeneration. The species is obligatory diplomatic apomítica. The transferability of microsatellite markers is conferred to species related, because of the sequences that precede the microsatellite regions, to be highly conserved among species of the same genus or of the same family. Although there are still no microsatellite primers developed for M. albicans, the primers developed for M. calvencens can be used as an alternative for the study of the genetic diversity of this species. The objective of this work was to evaluate the transferability potential of M. calvecens microsatellite markers for M. albicans. Total M. albicans DNA was extracted from the leaf tissue with the DNeasy Plant kit and quantitated with the use in NanoDrop (Thermo Fisher). The selected microsatellite primers (loci: B2, B9, B102, B109, B117, C103, D101, D114 and D118) developed for M. calvences were previously tested in 6 M. albicans individuals from a population of the Ecological Station of the Panga, Uberlândia-MG. The fragments were amplified in PTC-200 (MJ Research) thermocycler with different annealing temperatures, and subsequently the tests were performed with 20 individuals from a population. The amplification products were separated by electrophoresis on 3% agarose gel with the voltage of 110 V for 2 hours and 30 minutes, then photodocumented. The gels presented bands within the expected molecular weight pattern and occasionally nonspecific bands. We obtained 88% transferability of primers tested in M. albicans and evidences of heterozygosity and polyploidy effect.