Superfícies de implantes dentários e acetilação de Histona 3: efeitos na modulação óssea em condições de normalidade e osteoporose

Abstract

The number of elderly patients seeking dental implant treatment has increased in recent years. At the same time, more of these patients are expected to suffer from one or more chronic metabolic diseases, such as osteoporosis, that affect bone healing and may lead to more implant failures. This study aimed to characterize bone modulation and gene expression in relation to different surfaces of dental implants and Histone 3 acetylation through the pharmacological use of Vorinostat (HDACi) in conditions of normality and submitted to osteoporosis. This study was divided into three specific objectives; Specific objective 1: to evaluate the effect of a hydrophilic treatment surface on osteoblast differentiation and its effects on osseointegration. Specific objective 2: to determine the pattern of bone formation and gene expression on two different surfaces of titanium implants (hydrophobic and hydrophilic) in an osteoporotic model. Specific objective 3: to investigate global modifications in chromatin present in the cell nucleus mediated by titanium nano surface and pharmacological influence of histone acetylation influences the differentiation of mesenchymal stem cells, especially under conditions of osteoporosis. Due to the increased expression of genes related to osteogenic differentiation and to the significant amount of calcium and bone volume directly formed on the surface of SAE-HD dental implants, the choice of hydrophilic surface in osteoporotic situations can be significantly considered in order to improve process of osseointegration. Hydrophilic and nanoscale surfaces, as well as histone-mediated Vorinostat, may play a key role in the differentiation of mesenchymal stem cells into osteoblasts, especially under osteoporotic conditions. This is due to osteoconductive properties and the possibility of improving biological events occurring at the bone/implant interface.