Implementação da espectroscopia no infravermelho por transfomada de fourier com modo de reflexão total atenuada (atr-ftir) para diagnóstico salivar de asma

Abstract

Asthma is an extremely common condition with a global prevalence of ~ 10%. This condition develop symptoms which have a broad differential diagnosis. The gold standard protocol to diagnose asthma needs spirometrical analysis, so when it is not accessible the diagnosis are often poorly available to primary care physicians. ATR-FTIR is a rapid, free-label, non-destrutive and high-accurated platform that can analyse non-invasive samples of saliva. The present work aims at the salivary profile in the ATR-FTIR spectroscopy to analyze the composition of saliva in asthmatic animals compared to non-asthmatic ones. Male Wistar rats (mean of 350g) were divided into two groups: Non-Asthmatics treated with saline (SAL - n = 7) and Asvalids treated with ovalbumin (OVA - n = 7). Asthma was induced through ovalbumine and aluminum alloxide (AL) (OH) 3 (s.c.) on days 0, 7 and 14. On days 21, 22 and 23, OVA animals were observed to be antigenic with intranasal Ovalbumin. After 48 hours from the end of the game, the animals were anesthetized to collect saliva by pilocarpine and analyzed in FTIR. CEUA: 144/16 Asthmatic animals of the OVA group presented changes in the peak frequency 943cm-1, in the ROC curve with 100% and specificity of 71.43%. In this way, it is perceived that it can promote changes in saliva, which may be used to salivary diagnosis od asthma.