Produção das interleucinas 6 e 12 em culturas de endométrios caninos ex vivo com e sem inflamação desafiadas com lipopolissacarídeo

Abstract

The interleukins 6 (IL6) and 12 (IL12B) are proinflammatory cytokines that stimulate the synthesis of other inflammatory mediators and promote the activation and maturation of defense cells. Pathogen-Associated Molecular Patterns (PAMPs), such as lipopolysaccharides (LPS), bind to toll-like receptors (TLR), activating pathways that trigger the release of cytokines and other inflammatory molecules which participate together in the immune response against infectious agents. In bitches, some uterine conditions such as endometritis, cystic endometrial hyperplasia and pyometra have great clinical relevance. The synthesis of IL6 and IL12B in ex vivo canine endometrium cultures challenged with LPS was evaluated by sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The samples were histologically classified into four groups: without inflammation, acute inflammation, subacute inflammation and chronic inflammation. Results revealed that the challenge with LPS did not trigger a significative accumulation of IL12B and especially IL6 in the supernatant of inflamed ex vivo canine endometrium cultures, what can indicate that clinically healthy bitches with inflammatory infiltrates in endometrium may present a compromised uterine innate immune response against bacterial infection, contributing to the establishment of pyometra.