Inativação fotodinâmica de Saccharomyces cerevisiae utilizando rosa de bengala adsorvido em quitosana

Abstract

Chitosan is a natural biopolymer obtained from the chemical desacetylation of chitin. The interest in research for new applications of this polysaccharide allows biological and physical chemical characteristics such as biocompatibility, biodegradability, drug formation and mast cell nanocarrier may be different for photoinactivation processes of microorganisms. The photoactivation process is based on the topical or systemic administration of a non-toxic dye sensitive to light, then on low dose irradiation with suitable wavelength visible light. The present work evaluated the antibacterial of chitosan adsorption of xanthene Bengal Rose (RB), in relation to the effectiveness of the antibacterial of the dye in solution. The spatialces cerevisiae is an oracle obliterable the yeast Saccharomyces Cerevisiae. The spectral spectral spectral spectroscopy of ultraviolet and visible spectral region (UV-vis), obtaining the maximum dye wave size for the production of the irradiation system, consisting of LED 96 in the green color of the maximum power of 550 nm from the photosensitizer, the pink dye of Bengal. . Microscopic images show that the dye for its free form, relatively attached to the polysaccharide, does not have the process of metachromasia. The free dye photoinitiation tests were more than the applied dose (12.5 μM) of the dye was most effective in inhibiting the growth of viable Colony Forming Units (CFU’s). The adsorption was carried out during the dialysis process, being carried out in 48 hours to select the ideal time of adsorption of the dye to the chitosan biopolymer. The shorter dialysis time is more effective for biopolymer / dye aggregation, but there is an interaction between them in inhibiting the growth rate of microorganisms